Rapid Detection of Escherichia coli by Using Antibody‐Conjugated Silver Nanoshells
- 30 January 2006
- Vol. 2 (3) , 335-338
- https://doi.org/10.1002/smll.200500286
Abstract
Silver shells of 20 nm thickness have been deposited on silica particles of 200 nm diameter with narrow size distribution. Silver nanoshells dispersed in water exhibit a strong surface plasmon resonance band at 443 nm. This band was found to be very sensitive to rabbit immunoglobulin G antibodies, which were anchored on the nanoshells. These in turn could be utilized to detect the presence of small (≈5) to large numbers (≈109) of Escherichia coli in water. The protocol presented here proves to be a specific, rapid, reliable, and inexpensive method to detect E. coli.Keywords
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