Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
- 1 December 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (12) , 6260-6264
- https://doi.org/10.1073/pnas.76.12.6260
Abstract
The avian sarcoma virus [ASV] transforming gene product pp60src was partially purified using ion exchange or immunoaffinity chromatography. These preparations contain a cyclic AMP-independent protein kinase activity capable of transferring radiolabel from [.gamma.-32P]ATP to immune rabbit Ig[immunoglobulin]G, casein and the H chain purified from normal rabbit IgG. The casein kinase activity is specifically inhibited by anti-pp60src IgG. Comparison of the pp60src-protein kinase isolated from cells transformed by a wild-type ASV or by an ASV temperature-sensitive transformation mutant revealed that the latter product had a half-life of 3 min at 41.degree. C whereas that of the wild-type product was 20 min.This publication has 18 references indexed in Scilit:
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