STIMULATION OF GLYCOLYSIS BY PLACENTAL POLYPEPTIDES AND INHIBITION BY DURAMYCIN

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (4) , 1364-1367
Abstract
Placental polypeptides present in crude preparations of transforming growth factor stimulate glycolysis when added to quiescent 3T3 [mouse fibroblast] cells, normal rat kidney and chick embryo fibroblasts. The stimulation was apparent over a time period of at least 90 min and was seen at glucose concentrations ranging from 1-30 mM. Duramycin, an antibiotic isolated from Streptomyces cinnamomeus, inhibits the polypeptide-stimulated and nonstimulated glycolysis of intact cells, since it permeabilizes cells to Pi and nucleotides. However, duramycin also inhibits the Na+-K+-ATPase as well as the ouabain-insensitive Mg2+-ATPase of plasma membranes. Duramycin has no effect on glycolysis catalyzed by cell-free extracts of Ehrlich ascites tumor cells [mouse] in the presence of mitochondrial ATPase but partially inhibits glycolysis when ADP, and Pi are generated by ATPases of plasma membrane preparations.

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