Solubilization and partial characterization of adenosine binding sites from rat brainstem
- 11 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (1) , 93-97
- https://doi.org/10.1016/0014-5793(83)80683-8
Abstract
Binding sites for adenosine were solubilized from rat brainstem membranes with either sodium cholate, sodium deoxycholate or 3‐[3‐cholamidopropyl)dimethyl‐ammonio]‐1‐propanesulfonate. About 30% of the binding activity were released by these detergents as assayed by [3H]phenylisopropyladenosine (PIA) binding. Specific [3H]PIA binding to the solubilized fraction was saturable and was found to be a monophasic saturation profile. In contrast, [3H]PIA binding sites. By gel filtration on a Sepharose CL‐6B biphasic profile suggesting the presence of two binding sites. By gel filtration on a Sepharose CL‐6B column, the adenosine binding site—detergent complex was estimated to have app. M r 280 000 and r s = 5.4 nm.Keywords
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