Adhesion and migration of marrow‐derived osteoblasts on injectablein situcrosslinkable poly(propylene fumarate‐co‐ethylene glycol)‐based hydrogels with a covalently linked RGDS peptide

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Abstract
Marrow‐derived osteoblasts were cultured on poly(propylene fumarate‐co‐ethylene glycol) (P(PF‐co‐EG)) based hydrogels modified in bulk with a covalently linked RGDS model peptide. A poly(ethylene glycol) spacer arm was utilized to covalently link the peptide to the hydrogel. Three P(PF‐co‐EG) block copolymers were synthesized with varying poly(ethylene glycol) block lengths relative to poly(ethylene glycol) spacer arm. A poly(ethylene glycol) block length of nominal molecular weight 2000 and spacer arm of nominal molecular weight 3400 were found to reduce nonspecific cell adhesion and show RGDS concentration dependent marrow‐derived osteoblast adhesion. A concentration of 100 nmol/mL RGDS was sufficient to promote adhesion of 84 ± 17% of the initial seeded marrow‐derived osteoblasts compared with 9 ± 1% for the unmodified hydrogel after 12 h. Cell spreading was quantified as a method for evaluating adhesivity of cells to the hydrogel. A megacolony migration assay was utilized to assess the migration characteristics of the marrow‐derived osteoblasts on RGDS modified hydrogels. Marrow‐stromal osteoblasts migration was greater on hydrogels modified with 100 nmol/mL linked RGDS when compared with hydrogels modified with 1000 nmol/mL linked RGDS, while proliferation was not affected. These P(PF‐co‐EG) hydrogels modified in the bulk with RGDS peptide are potential candidates as in situ forming scaffolds for bone tissue engineering applications. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 260–270, 2003

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