Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications
Open Access
- 14 September 2010
- journal article
- Published by Springer Nature in BMC Research Notes
- Vol. 3 (1) , 239
- https://doi.org/10.1186/1756-0500-3-239
Abstract
A large portion of tissues stored worldwide for diagnostic purposes is formalin-fixed and paraffin-embedded (FFPE). These FFPE-archived tissues are an extremely valuable source for retrospective (genetic) studies. These include mutation screening in cancer-critical genes as well as pathogen detection. In this study we evaluated the impact of several widely used DNA extraction methods on the quality of molecular diagnostics on FFPE tissues.Keywords
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