Identification of the Functionally Active Methanotroph Population in a Peat Soil Microcosm by Stable-Isotope Probing
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Open Access
- 1 March 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (3) , 1446-1453
- https://doi.org/10.1128/aem.68.3.1446-1453.2002
Abstract
The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing. “Heavy” 13 C-labeled DNA, produced after microbial growth on 13 CH 4 , was separated from naturally abundant 12 C-DNA by cesium chloride density gradient centrifugation and used as a template for the PCR. Amplification products of 16S rRNA genes and pmoA , mxaF , and mmoX , which encode key enzymes in the CH 4 oxidation pathway, were analyzed. Sequences related to extant type I and type II methanotrophs were identified, indicating that these methanotrophs were active in peat exposed to 8% (vol/vol) CH 4 . The 13 C-DNA libraries also contained clones that were related to β-subclass Proteobacteria , suggesting that novel groups of bacteria may also be involved in CH 4 cycling in this soil.Keywords
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