Modulation of 72-Kilodalton Type IV Collagenase (Matrix Metalloproteinase-2) by Ascorbic Acid in Cultured Human Amnion-Derived Cells1
Open Access
- 1 August 1998
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 59 (2) , 326-329
- https://doi.org/10.1095/biolreprod59.2.326
Abstract
Extensive research has been done to investigate the effects of nutrients on placental and fetal development. It is now evident that environmental factors such as diet may exert a profound effect on gene expression during pregnancy. A low intake of vitamin C during pregnancy has been linked to a higher risk of premature rupture of the membranes (PROM) because of its well-known role in collagen biosynthesis. Here we report a new effect of ascorbic acid acting as a modulator of the 72-kDa type IV collagenase (matrix metalloproteinase-2; MMP-2). MMP-2 expression/activity is down-regulated by vitamin C in human amnion cultured cells. The regulatory effect is exerted at the transcriptional level and is specific for MMP-2. Matrix metalloproteinases are implicated in tissue remodeling, and our results allow us to suggest a molecular mechanism that relates poor availability of vitamin C during pregnancy and the development of PROM.Keywords
This publication has 11 references indexed in Scilit:
- Increased matrix metalloproteinase activity and reduced tissue inhibitor of metalloproteinases-1 levels in amniotic fluids from pregnancies complicated by premature rupture of membranesAmerican Journal of Obstetrics and Gynecology, 1996
- 92-kDa Gelatinase (Matrix Metalloproteinase-9) is Induced in Rat Amnion Immediately Prior to Parturition1Biology of Reproduction, 1995
- [28] Gelatinases A and BPublished by Elsevier ,1995
- Premature Rupture of Amniotic Membranes as Functional Assessment of Vitamin C Status during PregnancyAnnals of the New York Academy of Sciences, 1993
- Risk factors for preterm premature rupture of fetal membranes: A multicenter case-control studyAmerican Journal of Obstetrics and Gynecology, 1990
- Importance of vitamin C in maintenance of the normal amnion: an experimental studyPlacenta, 1986
- Restriction fragment length polymorphism associated with the pro alpha 2(I) gene of human type I procollagen. Application to a family with an autosomal dominant form of osteogenesis imperfecta.Journal of Clinical Investigation, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- The mechanical properties of prematurely and non—prematurely ruptured membranes Methods and preliminary resultsAmerican Journal of Obstetrics and Gynecology, 1976
- FUNCTION OF ASCORBIC ACID IN COLLAGEN METABOLISMAnnals of the New York Academy of Sciences, 1975