Genomic and proteomic profiling of responses to toxic metals in human lung cells.
- 1 May 2003
- journal article
- Published by Environmental Health Perspectives in Environmental Health Perspectives
- Vol. 111 (6) , 825-835
- https://doi.org/10.1289/ehp.111-1241504
Abstract
Examining global effects of toxic metals on gene expression can be useful for elucidating patterns of biological response, discovering underlying mechanisms of toxicity, and identifying candidate metal-specific genetic markers of exposure and response. Using a 1,200 gene nylon array, we examined changes in gene expression following low-dose, acute exposures of cadmium, chromium, arsenic, nickel, or mitomycin C (MMC) in BEAS-2B human bronchial epithelial cells. Total RNA was isolated from cells exposed to 3 M Cd(II) (as cadmium chloride), 10 M Cr(VI) (as sodium dichromate), 3 g/cm2 Ni(II) (as nickel subsulfide), 5 M or 50 M As(III) (as sodium arsenite), or 1 M MMC for 4 hr. Expression changes were verified at the protein level for several genes. Only a small subset of genes was differentially expressed in response to each agent: Cd, Cr, Ni, As (5 M), As (50 M), and MMC each differentially altered the expression of 25, 44, 31, 110, 65, and 16 individual genes, respectively. Few genes were commonly expressed...Keywords
This publication has 48 references indexed in Scilit:
- Decreased DNA repair gene expression among individuals exposed to arsenic in United States drinking waterInternational Journal of Cancer, 2003
- Effects of arsenite on p53, p21 and cyclin D expression in normal human fibroblasts — a possible mechanism for arsenite’s comutagenicityMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2001
- Applications of gene arrays in environmental toxicology: fingerprints of gene regulation associated with cadmium chloride, benzo(a)pyrene, and trichloroethylene.Environmental Health Perspectives, 2001
- Association of fine particulate matter from different sources with daily mortality in six U.S. cities.Environmental Health Perspectives, 2000
- Nickel-Induced Plasminogen Activator Inhibitor-1 Expression Inhibits the Fibrinolytic Activity of Human Airway Epithelial CellsToxicology and Applied Pharmacology, 2000
- Human Lymphocyte Heme Oxygenase 1 as a Response Biomarker to Inorganic ArsenicBiochemical and Biophysical Research Communications, 1998
- Cadmium, Gene Regulation, and Cellular Signalling in Mammalian CellsToxicology and Applied Pharmacology, 1997
- Interaction of arsenic(III) with nucleotide excision repair in UV- irradiated human fibroblastsCarcinogenesis: Integrative Cancer Research, 1997
- The accumulation of nickel in human lungs.Environmental Health Perspectives, 1989
- The human 32‐kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenaseFEBS Letters, 1989