Anabolic effect of phosphogenistein and phosphodaidzein on bone components in rat femoral-metaphyseal tissues in vitro

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Abstract
The effect of phosphogenistein and phosphodaidzein, which are phosphorylated for the hydroxyl group (OH) at the 7-position of genistein and daidzein, on bone components was investigated. Femoral-metaphyseal tissues obtained from male rats (4 weeks old) were cultured for 24–72 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The presence of phosphogenistein (10−5 M) caused a significant increase in calcium content, alkaline phosphatase activity, and deoxyribonucleic acid (DNA) content in bone tissues cultured for 24 h. Phosphodaidzein (10−5 M) significantly elevated bone calcium and DNA content. These effects were completely prevented by the presence of cycloheximide (10−6 M), an inhibitor of protein synthesis. When femoral-metaphyseal tissues were cultured for 48 h in the presence of parathyroid hormone (1–34) (PTH; 10−8 M) or prostaglandin E2 (PGE2; 10−6 M), bone calcium content was significantly decreased. This decrease was significantly blocked by the presence of phosphogenistein (10−6 and 10−5 M) or phosphodaidzein (10−6 and 10−5 M). The presence of PTH (10−8 M) or PGE2 (10−6 M) caused a significant increase in glucose consumption and lactic acid production by bone tissues. These increases were significantly inhibited by the presence of phosphogenistein (10−5 M) or phosphodaidzein (10−5 M), indicating their inhibitory effect on bone resorption. The present study has demonstrated that both phosphogenistein and phosphodaidzein have an anabolic effect on bone metabolism in rat femoral-metaphyseal tissues in vitro.

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