A Mutant of Escherichia coli with Temperature-Sensitive Streptomycin Protein
- 1 March 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 65 (3) , 544-550
- https://doi.org/10.1073/pnas.65.3.544
Abstract
The temperature-sensitive mutation in our Escherichia coli strain C1714 appears to be in a ribosomal protein. The mutation maps at the streptomycin locus. Protein synthesis in intact cells stops immediately when the temperature is shifted from 30 to 42 degrees C. Analysis of polyribosome distributions after shift-up suggests that initiation of protein synthesis is defective at the high temperature. In vitro protein synthesis in extracts from C1714 is temperature sensitive when directed by natural mRNA, but not when directed by poly U.Keywords
This publication has 12 references indexed in Scilit:
- In vitro synthesis of T4 proteinsBiochemical and Biophysical Research Communications, 1969
- Rapid inhibition of polypeptide chain extension by streptomycin.Proceedings of the National Academy of Sciences, 1968
- Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis.Proceedings of the National Academy of Sciences, 1968
- Polyribosomes of Growing BacteriaScience, 1967
- Cell-free protein synthesis programmed with R17 RNA: Identification of two phage proteinsJournal of Molecular Biology, 1966
- STUDIES ON THE RIBOSOMES OF STREPTOMYCIN-SENSITIVE AND RESISTANT STRAINS OF ESCHERICHIA COLIProceedings of the National Academy of Sciences, 1964
- ON THE COLINEARITY OF GENE STRUCTURE AND PROTEIN STRUCTUREProceedings of the National Academy of Sciences, 1964
- Inhibition of polypeptide synthesis by streptomycinBiochemical and Biophysical Research Communications, 1962
- RIBOSOMAL LOCALIZATION OF STREPTOMYCIN SENSITIVITYProceedings of the National Academy of Sciences, 1962
- Transduction of linked genetic characters of the host by bacteriophage P1Virology, 1955