Gibberellic acid-induced secretion of hydrolases in barley aleurone layers

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Abstract
Activities of phosphatases in the aleurone layers of a huskless barley, Ehime-hadaka No. 1, were enhanced in the absence of gibberellic acid (GA3), while the enzyme secretion was absolutely dependent upon its presence. GA3 was required for both induction and secretion of a-amylase. The longer the preincubation of the tissue without GA3, the longer was the lag period before secretion of both a group of phosphatases and a-amylase. Changes in the fine structure of aleurone cells were also investigated. Characteristics of the phase transition from enzyme accumulation to enzyme secretion seemed to be a development of a bundled type of endoplasmic reticulum.