Purification and Characterization of a Lectin from the Mushroom, Flammulina veltipes
- 1 November 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 86 (5) , 1463-1468
- https://doi.org/10.1093/oxfordjournals.jbchem.a132664
Abstract
A lectin was purified to homogeneity from the mushroom, Flammulina veltipes by zinc acetate treatment and CM-cellulose column chromatography. Its molecular weight was estimated to be 20,000 by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain carbohydrate, half-cystine, methionine, or histidine. On gel filtration with Sepharose 6B in the presence of 6 M guanidine-HCl, the purified lectin dissociated into two nonidentical subunits, FVA-L (molecular weight, 12,000) and FVA-S (8,000). The hemagglutinating activity was retained only in the FVA-L subunit. The lectin is mitogenic with respect to mouse spleen lymphocytes.Keywords
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