N-formimidoyl-thienamycin activity against anaerobes: effect of the inoculum, pH and culture media
- 1 September 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 8 (3) , 213-218
- https://doi.org/10.1093/jac/8.3.213
Abstract
The effect of three different culture media, three medium pH values and three inoculum densities on in-vitro activity of thienamycin against 100 isolates of anaerobes was tested. All media gave similar results for 50 Bacteroides spp. (25 β-lactamase producers), 25 clostridia and 14 Peptococcus spp. MICs for 12 Peptostreptococcus spp. were three times higher in brain-heart agar than in Brucella or Wilkins-Chalgren Agar. Medium pH 6–8, and inoculum density between 103–107 did not affect MIC significantly. All Bacteroides strains were inhibited at 0·125 mg/l and β-lactamase production had no effect. All Clostridia strains and Peptococcus spp. were inhibited at 0·125 mg/l, while the Peptostreptococcus spp. needed 1 mg/l.Keywords
This publication has 2 references indexed in Scilit:
- Thienamycin, a new .BETA.-lactam antibiotic. I. Discovery, taxonomy, isolation and physical properties.The Journal of Antibiotics, 1979
- In Vitro Activity of ThienamycinAntimicrobial Agents and Chemotherapy, 1978