Intracellular replication of infectious pancreatic necrosis virus

Abstract
Deoxyribonucleic acid (DNA) synthesis was repressed in cells 4 h after infection with infectious pancreatic necrosis (IPN) virus as determined by scintillation counting. Ribonucleic acid (RNA) synthesis was stimulated beginning 4 to 5 h after infection and reached a peak at 6 h. No Feulgen-positive viral inclusions were observed and the occasional inclusions seen exhibited green fluorescence when stained with acridine orange. These results suggest that the nucleic acid of IPN is double-stranded RNA.

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