THE AMYLOID IN FAMILIAL AMYLOID CARDIOMYOPATHY OF DANISH ORIGIN IS RELATED TO PRE-ALBUMIN

  • 1 January 1985
    • journal article
    • research article
    • Vol. 60  (1) , 207-216
Abstract
Amyloid obtained from the myocardium of a patient (Han) with familial amyloid cardiomyopathy of Danish origin was studied. Gel filtration and electrophoresis of purified and denatured amyloid fibrils Han revealed various fractions ranging in MW from 40,000 to 8000 daltons. Amyloid Han and fractions reacted with an antiserum against amyloid Han showing a reaction to identity with each other; partial identity between Han and human pre-albumin was observed, while no reaction was seen with AA or AL proteins. Cardiac tissue sections from Han showed reactivity with antisera to amyloid Han, pre-albumin and protein AP, but not with anti-AA or anti-AL in indirect immunofluorescence. Amino acid composition and sequence studies of a protein fraction of amyloid Han with MW 15,000 daltons confirmed the structural relationship with pre-albumin.