HPLC Determination of Iodide in Scrum Using Paired Ion Chromatography with Electrochemical Detection

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Abstract
HPLC [high performance liquid chromatography] utilizing paired ion chromatography with electrochemical detection is used for the determination of iodide in serum. Serum samples are prepared by precipitation of protein, centrifugation and removal of interfering substances with a bonded phase column. The resulting sample is then analyzed. The method was successfully applied to human serum and gives data that agrees well with values obtained by methods. The method is accurate precise, and time conservative when compared to more classical methods. [This research has applications to thyroid studies.].