Effects of Pronase on Isolated Chloroplasts

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Abstract
Subjecting isolated spinach [Spinacia oleracea] chloroplasts to mild proteolysis (10 min incubation at 20.degree. C in 500 mg/ml pronase) caused chloroplast clumping, but did not affect their integrity as measured by their ability to carry out light stimulated, glycerate-3-phosphate-dependent O2 evolution. Transmission electron microscopy revealed no detectable differences between the control and treated plastids. Mild proteolysis inactivated exogenously added pyruvate kinase and should be a useful technique in certain enzyme distribution studies.