Fluorogenic Reagents for Amino Acids in High-Performance Liquid Chromatography, Phenanthraoxazolylphenylisothiocyanates.

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Abstract
Two Edman-type fluorogenic reagents, 3- and 4-(2-phenanthra[9′, 10′-d]oxazolyl)phenylisothiocyanates, were synthesized. The reaction conditions of amino acids with these reagents were investigated for the high-performance liquid chromatographic determination of amino acids. Both reagents reacted with the tested 21 amino acids in basic medium to form the corresponding coupled derivatives in good yields. These products were converted in an acidic medium to the thiohydantoin derivatives. The derivatives were separated by reversed phase chromatography with fluorescence detection. The detection limits of amino acids (S/N=3) were pico- or subpicomole level on column.

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