Cholesterol‐Rich Rabbit Serum Modulates β‐Adrenergic Receptor Density of Human Lymphocytes
- 1 April 1992
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 650 (1) , 239-244
- https://doi.org/10.1111/j.1749-6632.1992.tb49130.x
Abstract
The effect of in vitro treatment of human lymphocytes with rabbit cholesterol-rich serum (RCS) on the membrane microviscosity as well as on the beta-adrenergic receptor density has been investigated. RCS treatment of cells resulted in a 30% decrease of receptor density without any effect on membrane microviscosity. A complete recovery was observed incubating the RCS cells either with the "Active Lipids" (AL) or with heparin. The AL are a mixture of neutral lipids, phosphatidylcholine and phosphatidylethanolamine from hen egg yolk known to fluidify the cell membrane. The AL modified membrane microviscosity of control lymphocytes without altering their beta-receptor number. These observations support the proposition that beta-receptor density of human lymphocytes is not regulated by membrane microviscosity and suggest that probably low density lipoprotein-cholesterol complex is involved in such a regulation.Keywords
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