Isolation of type I and type II GABAA‐benzodiazepine receptors by immunoaffinity chromatography
Open Access
- 17 June 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 284 (1) , 15-18
- https://doi.org/10.1016/0014-5793(91)80750-w
Abstract
Anti‐peptide α1 (1–9) and anti‐peptide α3 (459–467) antibodies coupled to Affigel‐10 were used for the isolation of GABAA receptors containing the α1‐or α3‐subunit, respectively. Both types of GABAA receptors exhibited a high affinity for [3H]flunitrazepam, and binding of [3H]flunitrazepam was stimulated in the presence of GABA. GABAA receptors eluted from the anti‐peptide α1 (1–9) immunoaffinity column exhibited a high affinity and those from the anti‐peptide α3 (459–467) columns a low affinity for the type I benzodiazepine receptor‐selective ligand Cl 218872, indicating the enrichment of type I and type II GABAA‐benzodiazepine receptors, respectively.Keywords
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