Novel susceptibility locus for mouse hepatomas: evidence for a conserved tumor suppressor gene.
Open Access
- 1 November 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 6 (11) , 1070-1076
- https://doi.org/10.1101/gr.6.11.1070
Abstract
We have identified previously a putative tumor suppressor gene (TSG) locus at human chromosome (hchr) 7q31 showing that it is altered in a variety of human epithelial tumors. To determine whether this TSG is conserved in mice, we studied loss of heterozygosity (LOH) in chemically induced mouse liver adenomas. The LOH analysis was performed by polymerase chain reaction amplification of 17 (CA)n microsatellite repeats on mouse chromosome (mchr) 6 A2-C3. Ninety-six of 106 cases (90.6%) had LOH at D6Mit50, and 89.5% had LOH at D6Mit179. These two loci are 0.2 cM apart on mchr 6A2. Another high-LOH site was found in the C3 band. The high incidence of LOH in the 7q-homologous segment of mchr 6 indicates that the human TSG is conserved and is involved in the development of hepatomas.Keywords
This publication has 18 references indexed in Scilit:
- A genetic map of the mouse with 4,006 simple sequence length polymorphismsNature Genetics, 1994
- Genome Maps IV 1993. Wall ChartScience, 1993
- Chromosome 7q deletions: Observations on 13 malignant tumorsCancer Genetics and Cytogenetics, 1993
- Comparison of cytogenetic and restriction fragment length polymorphism analyses for the detection of loss of chromosome material in clonal hemopoietic disordersAmerican Journal of Hematology, 1993
- Cytogenetic studies of eight squamous cell carcinomas of the head and neck: Deletion of 7q, a possible primary chromosomal eventCancer Genetics and Cytogenetics, 1992
- Tumor Suppressor GenesScience, 1991
- Oncogenes and tumor-suppressor genes.Environmental Health Perspectives, 1991
- Oncogene Activation and HepatocarcinogenesisPathobiology, 1990
- Evidence for growth heterogeneity among foci with different phenotypes in the population of altered hepatocyte foci induced by a single neonatal treatment with carcinogenCarcinogenesis: Integrative Cancer Research, 1986
- Differential induction of sister-chromatid exchanges by benzo[a]pyrene in variant mouse hepatoma cellsMutation Research Letters, 1985