Chronic Use of Intracerebral Dialysis for the In Vivo Measurement of 3,4‐Dihydroxyphenylethylamine and Its Metabolite 3,4‐Dihydroxyphenylacetic Acid

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Abstract
The intracerebral dialysis technique was studied with a method in which the rat was directly connected to the HPLC equipment. The effect of three pharmacological treatments [perfusion of 60 mmol K+ or 5 × 10−5M (+)‐amphetamine or subcutaneous injection of 2 mg/kg (+)‐amphetamine] on the release of 3,4‐dihydroxy‐phenylethylamine (dopamine) and 3,4‐dihydroxyphenylacetic acid was followed over a period of 7 days. The marked rise of dopamine output seen after infusion of K+ had almost disappeared on day 3. Tissue reactions around the membrane presumably formed a barrier preventing K+ from reaching dopaminergic terminals. In contrast, the pronounced rise in dopamine level after amphetamine (infused as well as systemically administered) was still present (although diminished) 8 days after implantation. It is concluded that, with certain restrictions, brain dialysis of dopamine is still useful several days after implantation of the membrane.

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