Differential gene expression profiling of short and long term denervated muscle

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Abstract
SPECIFIC AIMSSkeletal muscle reinnervated after short periods of denervation recovers mass, form, and contractile function. In contrast, long-term denervation results in irreversible muscle impairment from permanent atrophy, fiber death, and fibrosis. We sought to investigate the molecular mechanisms underlying the irreversibility of the pathology of long-term denervated muscle. We performed transcriptional profiling of short- and long-term denervated muscle with cDNA microarrays and real-time RT-PCR. Analysis of the expression pattern of differentially regulated genes in specific gene ontology categories permitted delineation of signaling pathways and processes governing reversible and irreversible changes in short and long-term denervated muscle. We hypothesized that pathways unique to each of the two time points would be identified.PRINCIPAL FINDINGSMicroarray analysis of RNA from rat gastrocnemius muscle denervated for 1 or 3 months was performed. Contralateral unoperated gastrocnemius muscle in each ...