Molecular aspects of the binding of absorbed iron to transferrin
- 1 September 1982
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 52 (1) , 105-110
- https://doi.org/10.1111/j.1365-2141.1982.tb03866.x
Abstract
To study the molecular aspects of the binding of absorbed Fe to plasma transferrin, 59Fe with high specific activity was administered via intragastric tube to Fe-deficient rabbits. The distribution of the absorbed 59Fe among the molecular forms of Fe-transferrin was analyzed using urea-polyacrylamide gel electrophoresis. Absorbed Fe was bound to circulating transferrin 1 atom at a time. In 4 of 5 animals, absorbed Fe was predominantly bound to the site in the N-terminal domain of the protein. Thus, the 2 sites of transferrin may differ in their ability to load absorbed Fe.This publication has 12 references indexed in Scilit:
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