Incorporation of 35S-Methionine or 35S-Cystine into Polypeptide Protease Inhibitor in the Rabbit Skin with Healing Inflammation (Preliminary Report)

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Abstract
The specific inhibitor of SH-dependent skin protease responsible for the development of Arthus-type inflammation was isolated from the skin site and highly purified by chromatography. The gradual cessation of the inflammatory process was associated with a local increase of the inhibitor. The inhibitor (polypeptide) behaved as a homogeneous substance in electro-phoresis and ultracentrifugation. The constituent amino-acids of the polypeptide were: Cys, Met, Asp, Thr, Ser, Glu, Pro, Ala, Gly, Val, Leu, Ileu, Phe, Lys, His, Arg and HO-pro. The labeled amino-acids (35s-methionine or 35S-cystine) given intravenously were incorporated into the inhibitor; approximately 87% of radioactivity in the hydrolyzed inhibitor was found in methionine and approximately 13% in cystine. The observations suggested possible synthesis of this inhibitor in the inflamed skin site.