FAST AND SLOW SKELETAL-MUSCLES - SIMULTANEOUS INVITRO STUDY

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 57  (5) , 223-227
Abstract
Simultaneous study of the function of fast and slow twitch skeletal muscles in the same in vitro preparation has obvious advantages. Equipment, method and technique are described for study of the soleus (slow) and extensor digitorum longus (fast) muscles from the mouse, and the problems involved in preparing and standardizing these studies are discussed. For nearly maximum function to be obtained from both muscles, careful attention must be given to the stimulus parameters used. Three separate studies were necessary to determine the appropriate stimulus duration, frequency and rest interval between the stimulus trains. For study of tetanic tension and rate of tension development of these muscles from the C57 NAB strain of mice at 20.degree. C the most satisfactory stimulus parameters are: duration 2 ms, frequency 100 pulses/s and duration of the stimulus train 700 ms. Rest interval between stimulus trains was 2 min as problems developed with rest intervals less than 2 min or greater than 4 min.

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