Globin Synthesis in Bone Marrow Cells of Patients with Sickle Cell Anemia and βO-Thalassemia: Contamination of the β-Chain with Non-Globin Proteins
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 3 (2-3) , 175-183
- https://doi.org/10.3109/03630267908998912
Abstract
Chromatographic separation of labeled globin chains was performed in stroma-free hemolysates prepared from peripheral blood and bone marrow cells of 11 patients with .beta.O-thalassemia and 2 patients with sickle cell anemia. A small radioactivity peak, slightly preceding the .beta.-chain and more prominent in bone marrow cells, was often observed. This peak, which represents synthesis of non-globin proteins, did not exceed 5% of the radioactivity incorporated in the .alpha.-chain. Contamination of the .beta.-chain with non-globin proteins undoubtedly occurs, but its extent is insufficient to explain the different synthetic ratios which were repeatedly observed in peripheral blood and in bone marrow cells of patients with heterozygous .beta.-thalassemia.This publication has 12 references indexed in Scilit:
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