COMPARISON OF COLONY STIMULATING ACTIVITIES SECRETED INTO MOUSE LUNG CONDITIONED MEDIUM IN THE PRESENCE AND ABSENCE OF LITHIUM-CHLORIDE
- 1 January 1982
- journal article
- research article
- Vol. 10 (1) , 123-129
Abstract
Inclusion during the process of secretion of granulocyte-macrophage colony stimulating activity (CSA) into mouse lung conditioned medium resulted in an increased production of CSA. CSA produced in the presence of LiCl was compared to the CSA produced in its absence by means of ammonium sulfate precipitation, Sephadex G-1CO (S) chromatography, concanavalin A-Sepharose chromatography and polyacrylamide gel electrophoresis. The 2 activities behaved identically, indicating that Li-induced increase inCSA does not involve a qualitative change in the molecule of the colony stimulating factor [CSF], but relfects an increased production (or release) of a CSF molecule similar to the one produced in its absence.This publication has 2 references indexed in Scilit:
- Serum of lipopolysacharide-treated mice contains two types of colony-stimulating factor, separable by affinity chromatographyJournal of Cellular Physiology, 1980
- Similar molecular properties of granulocyte-macrophage colony-stimulating factors produced by different mouse organs in vitro and in vivo.Journal of Biological Chemistry, 1979