Rapid, High-Level Transient Expression of Papillomavirus-Like Particles in Insect Cells
Open Access
- 1 May 1996
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 20 (5) , 890-895
- https://doi.org/10.2144/96205rr02
Abstract
Empirical scanning of natural or engineered peptide sequences for functional residues is inherently dependent upon efficient expression of large numbers of individual sequence variants to assay their relative functional potency. The insect baculovirus system has been widely used for expression of viral coat proteins, but it generally requires prior isolation and expansion of a plaque-purified recombinant viral stock to generate useful quantities of selfassembled virus-like particles. In search of a more rapid means of expression of analytical levels of the L1 coat protein of cottontail rabbit and human type 11 papillomaviruses, we found that even brief transient cotransfection of insect cells with baculovirus plasmid transfer vectors and viral DNA yielded assembled particles that were immunologically indistinguishable from particles obtained with plaque-purified viral stocks. Within six days of plasmid/viral DNA cotransfection of Sf9 cells, at least 1–2 mg of assembled L1 particles/100-mm plate could be d...Keywords
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