Simple, rapid method for converting a peptide from one salt form to another
- 1 July 1987
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 30 (1) , 40-43
- https://doi.org/10.1111/j.1399-3011.1987.tb03310.x
Abstract
A method for converting peptide trifluoroacetate salts to the corresponding acetate salts has been developed. The procedure involves reversed phase HPLC with a volatile buffer system. The method is exemplified by the conversion of Growth Hormone-Release Factor, GRF (1-44)-NH2 trifluoroacetate to the acetate which was achieved in > 95% recovery. Extensive analytical studies of the product confirmed the absence of trifluoroacetate and that the acetate salt was obtained without degradation. This procedure is expected to be generally applicable to other peptides and to other salt form conversions.Keywords
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