Lactoferrin receptors in normal and leukaemic human blood cells

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Abstract
The binding of 125I-lactoferrin to a variety of peripheral blood cells was examined. In the concentration range from 10-9 mol/l to 10-6 mol/l a receptor-like binding of lactoferrin was observed in monocytes as well as in polymorphonuclear leukeocytes. At the low concentrations of lactoferrin in plasma (about 10-9 mol/l) the cellular binding to monocytes was about 10 times higher than the binding to polymorphonuclear leukocytes and lymphocytes. A major result from a kinetic analysis was a lower apparent binding affinity to polymorphonuclear leukocytes (Kd about 2 .times. 10-7 mol/l) than to lymphocytes and monocytes (K d about 2 .times. 10-8 mol/l). Studies in leukemic cells showed that lymphocytes from patients with chronic lymphocytic leukemia bound lactoferrin to the same small extent as normal lymphocytes. A larger component of binding with high affintiy (Kd about 2 .times. 10-8 mol/l) could be demonstrated to lymphoblasts as well as to myeloblasts.