UNTERSUCHUNGEN ÜBER DIE 17β-HYDROXYSTEROID-DEHYDROGENASE DER MENSCHLICHEN NEBENNIERENRINDE
- 1 January 1964
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 45 (1) , 47-54
- https://doi.org/10.1530/acta.0.0450047
Abstract
17[beta]-Hydroxysteroid dehydrogenases have been found to be present in the cytoplasma fractions of normal and hyperplastic human adrenal cortex. A 10-fold purification of the enzymes was achieved by precipitation with ammonium sulphate (40-80% saturation). With testosterone as substrate both enzymes possess similar specific activities; NAD as well as NADP can act as cofactors. The 17[beta]-hydroxysteroid dehydrogenase obtained from the hyperplastic adrenal cortex shows a pH optimum at 8.4; the Michaelis-Menten constant for testosterone was found to be 9.8 x 10-4 [image]. The enzymatic activity is markedly inhibited by SH blocking agents.Keywords
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