Fine mapping of HIV-1 Nef-epitopes by monoclonal antibodies
- 1 March 1993
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 128 (1-2) , 81-95
- https://doi.org/10.1007/bf01309790
Abstract
A panel of newly isolated murine monoclonal antibodies is described which are specific for the Nef protein of the human immunodeficiency virus type 1 (HIV-1). Epitope mapping using recombinant Nef-related proteins, synthetic peptides and lipopeptides showed 3 independent antigenic determinants located within the regions of amino acids 83–93, 175–190 and 86–166 of the Nef protein. None of the monoclonal antibodies reacted with recombinant Nef proteins of HIV-2.Keywords
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