DICUMAROL-2-C14: SYNTHESIS AND METABOLISM STUDIES
- 1 April 1952
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Chemistry
- Vol. 30 (4) , 245-250
- https://doi.org/10.1139/v52-035
Abstract
Dicumarol (3,3′-methylene bis(4-hydroxycoumarin)) has been synthesized labeled with C14 in the 2-position. Starting with active barium carbonate of specific activity 1.6 µc.per mgm., the intermediate compounds diethyl carbonate-1-C14 and 4-hydroxycoumarin-2-C14 were prepared. Subsequent reaction of the latter compound with aqueous formaldehyde yielded dicumarol-2-C14. The specific activity of the final product, once recrystallized from cyclohexanone, was 4.3 × 105 counts per min. per mgm. corrected for geometry and absorption. The expired carbon dioxide from a mouse injected with this C14-labeled product was collected and found to contain no radioactivity. This indicated that, in this animal, the 2-carbon of dicumarol does not enter into a metabolic process whereby it is expired as carbon dioxide.This publication has 1 reference indexed in Scilit:
- Dicumarol Labelled with C14.Experimental Biology and Medicine, 1950