THE CYTOTOXIC AND MUTAGENIC EFFECTS OF UVA RADIATION ON L5178Y MOUSE LYMPHOMA CELLS
- 1 July 1986
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 44 (1) , 53-57
- https://doi.org/10.1111/j.1751-1097.1986.tb03563.x
Abstract
Abstract— A broad‐band UVA source that emits primarily350–400 nm radiation and no measurable radiation below 340 nm was used to test toxicity and mutagenicity at the thymidine kinase locus in L5178Y, subclone 3.7.2C (TK+/‐) mouse lymphoma cells. Cells were exposed to a fluence of 0 to 80 × 104 J/m2. The relationship between UVA fluence and survival was found to have a shoulder region followed by an exponential decrease in survival at higher fluence levels. An exposure‐dependent increase in mutation was observed with increasing fluences from 0 to about 60 × 104 J/m2. An approximately 3‐ to 4‐fold increase in mutations (trifluorothymidine resistance) over unexposed, control cells was seen at a fluence that resulted in 90% cell killing. We conclude that UVA radiation is a mutagen in the L5178Y mouse lymphoma cells used in this study.This publication has 34 references indexed in Scilit:
- THE REPAIR OF DNA STRAND BREAKS IN HUMAN LYMPHOCYTES EXPOSED TO NEAR UV-RADIATION (UVA) AND FAR UV-RADIATION (UVC)Photochemistry and Photobiology, 1985
- Mutagenic action of monochromatic UV radiation in the solar range on human cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1984
- Mutagenicity of 8-methoxypsoralen and long-wave ultraviolet irradiation in diploid human skin fibroblasts an imported risk estimate in photochemotherapyMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Ultraviolet wavelength regions implicated in toxic and mutagenic effects of broad spectrum radiation from fluorescent lamps on L5178Y mouse lymphoma cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Mutagenicity of 8-methoxypsoralen and long-wave ultraviolet irradiation in V-79 Chinese hamster cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1979
- Comparison of the lethal and mutagenic effects of gold and white fluorescent lights on cultured mammalian cellsMutation Research/Environmental Mutagenesis and Related Subjects, 1978
- Mutagenic interaction between near-(365 nm) and far-(254 nm) ultraviolet radition in repair-proficient and excision-deficient strains of Escherichia coliMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Toxicity and mutagenicity of radiation from fluorescent lamps and a sunlamp in L5178Y mouse lymphoma cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
- Mutagenesis by fluorescent light in mammalian cell culturesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Recent developments with the L5178Y TK heterozygote mutagen assay system.Environmental Health Perspectives, 1973