Whole Mounts of Rabbit Lens Epithelium for Cytological Study

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Abstract
The eye globe is cut equatorially just posterior to the equator and the anterior part fixed in acetic-acid:akohol (1:3) for 24 hours. After washing, the lens is removed, hydrolyzed, and stained by the Feulgen technic. In the SO2 wash solution, the lens epithelium is dissected free of the lens in one piece under a binocular microscope and laid on a cover slip, cell side down. It is flattened, after radial incisions, by absorbing water around the edge, and inverted on to a drop of glychrogel on a slide. Cell and nuclear structure are observed with the aid of phase contrast.

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