Orthodox Behaviour of Oil Palm Seed and Cryopreservation of the Excised Embryo for Genetic Conservation

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Abstract
Desiccation experiments have shown the oil palm seed to be orthodox, not recalcitrant, in character. There is a significant difference in water content between the whole seed and the embryo which is maintained despite desiccation, and results in the failure of sub-zero storage of whole seeds. The successful recovery of viable, excised embryos from liquid nitrogen, and their subsequent regrowth in vitro, suggests a practical technique for the long term conservation of the genetic resources of the species.

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