Submicroscopical changes in Klebsiella pneumoniae cells treated with concentrated sucrose and polyethylene glycol 400 solutions

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Abstract
This study reports the extent and character of plasmolysis and other morphological changes as shown by electron microscopy in a strain of Klebsiella pneumoniae and with sucrose or polyethylene glycol 400 (PEG‐400) as the plasmolysing agent at a water activity of 0.935.Both solutes produced severe plasmolysis in K. pneumoniae cells; PEG‐400 also caused some cell wall collapse and fingerlike extrusions to emerge from the bacterial cell.