Induction of Accelerated Senescence by γ Radiation in Human Solid Tumor-Derived Cell Lines Expressing Wild-Type TP53

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Abstract
Mirzayans, R., Scott, A., Cameron, M. and Murray, D. Induction of Accelerated Senescence by γ Radiation in Human Solid Tumor-Derived Cell Lines Expressing Wild-Type TP53. Radiat. Res. 163, 53–62 (2005). Recent studies have demonstrated that p21WAF1 (now known as CDKN1A)-dependent and -independent accelerated senescence responses are a major determinant of the sensitivity of cancer cells to chemotherapeutic agents. The objective of the present study was to determine whether human solid tumor-derived cell lines that express wild-type TP53 can exhibit levels of CDKN1A induction after exposure to ionizing radiation that are sufficient to activate the accelerated senescence program. Exposure to 60Co γ radiation (≤8 Gy) triggered accelerated senescence in all five TP53 wild-type tumor cell lines examined, albeit to differing degrees. Three of the TP53 wild-type tumor cell lines, HCT116, A172 and SKNSH, activated the TP53 signaling pathway similarly to normal human fibroblasts, as judged by the nuclear ...