Abstract
Semen was collected from 33 sexually mature walleye, Stizostedion vitreum vitreum (Mitchill), captured in 3 northeastern Colorado reservoirs. Physical and chemical measurements were made on each sample of semen. The physical properties measured and the mean values of each were: total length (of fish), 555.3 mm; weight (of fish), 1,694.6 g; spermatocrit (percentage of packed cells to total volume), 55%; freezing point depression (Δ °) ‐0.489; dry weight as % of wet weight, 20.8%, and ash weight as % of dry weight, 11.33%. Chemical analyses were run on individual samples of seminal plasma to determine pre‐ and post‐freezing levels of certain constitutents and to measure changes which occur following freezing. The values obtained in mg/100 ml were: Ca, 3.2 to 1.5; Cl, 467.5 to 427.9; Mg, 1.6 to 4.7; P, 5.3 to 51.9; K, 97.0 to 156.8; Na, 383.3 to 241.6; total protein, 2.6 to 5.9; urea N, 2.9 to 8.1; and uric acid, 3.3 to 11.1, respectively for pre‐ and post‐freezing levels. These properties were also measured in frozen and thawed sperm cell cytoplasm. Simple linear regression analysis was conducted on all variables measured. Chemical properties of fresh walleye seminal plasma are compared to values obtained by other authors for Atlantic salmon (Salmo salar, human, bull, fowl and dog seminal plasma.

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