Some Physiological Properties of Protoplasts Isolated from Maize and Tobacco Tissues2

Abstract
The stability and respiratory and permeability properties of protoplasts isolated from maize and tobacco tissues have been investigated. Oxygen uptake rates of maize leaf protoplasts showed the expected response to a variety of metabolic inhibitors. Studies of leakage from preloaded tissues during protoplast isolation and of phosphate and potassium uptake byprotoplasts indicated that isolation produced a marked increase in membrane permeability. Ageing of protoplasts for 24 h produced a marked change in the ability of maize leaf protoplasts to absorb potassium.

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