DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.
Open Access
- 1 November 1985
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 5 (11) , 3293-3296
- https://doi.org/10.1128/mcb.5.11.3293
Abstract
Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.Keywords
This publication has 21 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Molecular cloning of a human DNA repair geneNature, 1984
- Lack of induction of O6-methylguanine-DNA methyltransferase in mammalian cells treated with N-methyl-N' -nitro-N-nitrosoguanidineCarcinogenesis: Integrative Cancer Research, 1984
- Molecular identification of a human DNA repair gene following DNA-mediated gene transferNature, 1983
- Mechanism of action of the nitrosoureas—VBiochemical Pharmacology, 1983
- Pretreatment of normal human fibroblasts and human colon carcinoma cells with MNNG allows chloroethylnitrosourea to produce DNA interstrand crosslinks not observed in cells treated with chloroethylnitrosourea aloneCarcinogenesis: Integrative Cancer Research, 1983
- Removal of O6-methylguanine from DNA of normal and xeroderma pigmentosum-derived lymphoblastoid linesNature, 1981
- DNA cross-linking and monoadduct repair in nitrosourea-treated human tumour cellsNature, 1980
- Evidence for an adaptive DNA repair pathway in CHO and human skin fibroblast cell linesNature, 1980
- Transformation of mammalian cells with genes from procaryotes and eucaryotesCell, 1979