A Carotenoid Cleavage Dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation
Open Access
- 30 August 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 56 (420) , 2721-2731
- https://doi.org/10.1093/jxb/eri265
Abstract
A potential Carotenoid Cleavage Dioxygenase (CCD) gene was identified among a Vitis vinifera L. EST collection and a full-length cDNA (VvCCD1) was isolated. Recombinant expression of VvCCD1 confirmed that the gene encoded a functional CCD. Experimental evidence was obtained that VvCCD1 cleaves zeaxanthin symmetrically yielding 3-hydroxy-β-ionone, a C13-norisoprenoidic compound, and a C14-dialdehyde. Expression of the gene was studied by real-time PCR at different developmental stages of grape berries from Muscat of Alexandria and Shiraz cultivars. A significant induction of the gene expression approaching véraison was observed in both cultivars. In parallel, the C13-norisoprenoid level increased from véraison to maturity in both cultivars.Keywords
This publication has 28 references indexed in Scilit:
- Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (−)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berriesPhytochemistry, 2004
- Gradients in stomatal conductance, xylem sap ABA and bulk leaf ABA along canes of Vitis vinifera cv. Shiraz: molecular and physiological studies investigating their sourceFunctional Plant Biology, 2004
- Biochemical Properties of Purified Recombinant Human β-Carotene 15,15′-MonooxygenaseJournal of Biological Chemistry, 2002
- Biogeneration of C13-norisoprenoid compounds: experiments supportive for an apo-carotenoid pathway in grapevinesAnalytica Chimica Acta, 2002
- Co-oxidation of β-Carotene Catalyzed by Soybean and Recombinant Pea LipoxygenasesJournal of Agricultural and Food Chemistry, 1999
- ChloroP, a neural network‐based method for predicting chloroplast transit peptides and their cleavage sitesProtein Science, 1999
- Glycosidically Bound Norisoprenoids from Vitis vinifera cv. Riesling LeavesJournal of Agricultural and Food Chemistry, 1994
- Glycosidic Precursors of Varietal Grape and Wine FlavorPublished by American Chemical Society (ACS) ,1992
- Two-dimensional GC-DCCC analysis of the glycoconjugates of monoterpenes, norisoprenoids, and shikimate-derived metabolites from Riesling wineJournal of Agricultural and Food Chemistry, 1990
- Carotene cooxidation activity of tomato lipoxygenase‐2Molecular Nutrition & Food Research, 1988