Induction of mutation to ouabain resistance and of malignant transformation by chemicals in cloned mouse M2 fibroblasts
- 31 December 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 1 (3) , 215-218
- https://doi.org/10.1093/carcin/1.3.215
Abstract
Malignant transformation and mutation to ouabain resistance is induced in cloned M2 mouse fibroblasts in vitro by treatment with N-methyl-N''-nitro-N-nitrosoguanidine, the 3,4-diol of 7-methylbenz[a]anthracene and the 7,8-diol-9,10-oxide of benzo[a]pyrene. Transformation is more frequent than mutation; the ratio of transformation:mutation frequencies varied between 24 and 1118. The 9,10-diol-7,8-oxide derived from benzo[a]pyrene induced ouabain resistance without causing transformation. Induced ouabain-resistant clones did not behave like transformants and transformants did not exhibit ouabain resistance. The significance of these findings is discussed.This publication has 8 references indexed in Scilit:
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