Inoculation of loblolly pine seedlings with Fomes annosus in the greenhouse

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Abstract
A reliable method of inoculating loblolly pine seedlings with Fomes annosus under greenhouse conditions is described. Type of wound, wounding vs. nonwounding, and soil temperature influenced the number of pine seedlings killed. Inoculum weight and orientation of the inoculum block had less effect on mortality. No variation in virulence of eight isolates of the fungus was demonstrated. Seedling mortality occurred at soil temperatures from 10 to 30 °C, but the disease index was highest at 15–25 °C.

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