Absorption of Doxorubicin-Hydrochloride and Mitomycin-C After Instillation into Noninfected and Infected Bladders of Dogs

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Abstract
The degree of absorption of doxorubicin and mitomycin C [cytostatic agents] after intravesical instillation into noninfected or Staphylococcus aureus-infected bladders in beagle dogs was determined. The drug concentrations in the bladder wall were determined using diffusion chambers with permeable membranes. Two hours after end of instillation of 10 mg doxorubicin, a concentration of 1.4 ng/ml was measured in the bladder wall of noninfected animals and 3.75 ng/ml in that of infected animals (P < 0.05). The simultaneously measured serum concentration reached mean peak levels after 30 min. The concentrations in infected animals were 3 times higher (1.9 ng/ml) than in noninfected animals (0.6 ng/ml) (P < 0.05). After instillation of 1 mg/kg body weight mitomycin C the concentration in both groups of animals was < 0.06 .mu.g per ml. Doxorubicin concentrations were determined with a radioimmunoassay and mitomycin C with a micro-agar diffusion method.