Preparation and Validation of PCR-generated Positive Controls for Diagnostic Dot Blotting
- 1 July 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 44 (7) , 1578-1579
- https://doi.org/10.1093/clinchem/44.7.1578
Abstract
Allele-specific oligonucleotides (ASOs) are small, single-stranded nucleotide polymers (∼18–20 bases in length) of diagnostic utility given their ability to hybridize to single-stranded DNA target molecules in a sequence-specific, temperature-dependent manner. Accordingly, two target molecules differing in composition by a single base can be distinguished by hybridization with an ASO that is complementary to one of the target molecules but noncomplementary to the other by a single base. The latter target produces a single basepair-mismatched double strand that dissociates at a lower washing temperature than the fully complementary, double-stranded molecule.Keywords
This publication has 4 references indexed in Scilit:
- Direct detection of multiple point mutations in mitochondrial DNAClinical Chemistry, 1997
- Phylogenetic analysis of the mitochondrial genomes from Leber hereditary optic neuropathy pedigrees.Genetics, 1995
- A robotics-assisted procedure for large scale cystic fibrosis mutation analysisHuman Mutation, 1994
- Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.Proceedings of the National Academy of Sciences, 1983