Polymeric cryoproteetants in the preservation of biological ultrastructure
- 2 August 1977
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 110 (3) , 257-270
- https://doi.org/10.1111/j.1365-2818.1977.tb00036.x
Abstract
Two high molecular weight polymers, polyvinylpyrrolidone (PVP) and hydroxyethyl starch (HES), have been used as cryoproteetants for preparing specimens to be freeze fractured. Solutions of 25% (w/w) suppress the formation of intracellular ice in single cells and tissue blocks from both plants and animals to the extent that fine structural details of the cell can be elucidated. The mode of action of these cryoproteetants, together with the structures they reveal and the peculiar advantages attached to their use, is discussed.This publication has 29 references indexed in Scilit:
- Het mechanism of the protective action of glycerol against haemolysis by freezing and thawingPublished by Elsevier ,2003
- Polymeric cryoproteetants in the preservation of biological ultrastructureJournal of Microscopy, 1977
- Water transport and cell survival in cryobiological proceduresPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1977
- Aqueous glasses as matrices in freeze-fracture electron microscopyNature, 1976
- Freezing Injury and its Prevention in Living CellsAnnual Review of Biophysics and Bioengineering, 1974
- FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTSThe Journal of cell biology, 1972
- Recent progress in the freeze-etching techniquePhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971
- MEMBRANE SPLITTING IN FREEZE-ETCHINGThe Journal of cell biology, 1970
- Is there a Common Mechanism of Protection of Living Cells by Polyvinylpyrrolidone and Glycerol during Freezing?Nature, 1969
- Optimal Conditions and Comparative Effectiveness of Dimethyl Sulphoxide and Polyvinylpyrrolidone in Preservation of Bone MarrowNature, 1963