beta-globin dominant control region interacts differently with distal and proximal promoter elements.
Open Access
- 1 June 1990
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 4 (6) , 1007-1013
- https://doi.org/10.1101/gad.4.6.1007
Abstract
We have studied the interaction between the dominant control region (DCR) and the promoter of the human beta-globin gene. Expression analysis in MEL cells has revealed that the DCR contains a number of elements capable of replacing the upstream (-250 to -100) erythroid-specific region of the promoter. The DCR strongly stimulates expression from a promoter possessing only a TATA box. However, this basic level of transcription is not induced upon erythroid differentiation of the cells. Mutational analysis of the minimal (-100, noninducible) promoter shows that only the combination of the DCR and the CAC/CCAAT elements provides erythroid-specific transcription. These regions act synergistically to produce full regulated expression during erythroid differentiation.This publication has 36 references indexed in Scilit:
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